Question 1

All of the following are requirements of a bacterial cloning vector EXCEPT:

Accepted Answer

A)  origin of replication.
B)  unique restriction enzyme sites.
C)  Ti plasmid.
D)  selectable markers.
E)  All of these are requirements of a bacterial cloning vector.F) All of the above
G) A) and D)

Question 2

A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site.After dideoxy sequencing, the gel pattern shown in this diagram is obtained.What was the sequence of the DNA strand that acted as the template in the sequencing reaction?

Accepted Answer

A)  5' GCTAGCA 3'
B)  5' ACGATCG 3'
C)  5' TGCTAGC 3'
D)  5' CGATCGT 3'
E)  None of the answers is correct.F) A) and B)
G) A) and C)

Question 3

The restriction enzyme AcyI cuts duplex DNA at the sequence 5' GPuCGPyC 3'
3' CPyGCPuG 5'
Where Pu = either purine and Py = either pyrimidine.On average, what is the length of DNA fragments in base pairs created by this enzyme? (Assume that the four bases are found in equal frequencies in this DNA sample.)

Accepted Answer

A)  48
B)  512
C)  4096
D)  256
E)  1024F) A) and E)
G) C) and E)

Question 4

How might RNAi be used to treat diseases in humans?

Accepted Answer

A)  siRNAs could be generated that would silence elevated levels of a harmful gene.
B)  An RNAi library could be generated for harmful genes that are expressed.
C)  RNAi probes could bind to good genes, and thus increase their expression.
D)  RNAi is a process that has the ability to amplify large amounts of therapeutic proteins.
E)  RNAi is not a process that could work in humans, as it is a process found only in plants.F) B) and E)
G) A) and D)

Question 5

Which of the following can be used for genetic engineering in plants?

Accepted Answer

A)  Ti plasmid
B)  Restriction enzymes
C)  Selectable markers
D)  Ti plasmid and restriction enzymes are both correct.
E)  All of the answers are correct.F) A) and D)
G) C) and E)

Question 6

Which of the following is NOT part of the normal process of cloning recombinant DNA in bacteria?

Accepted Answer

A)  Restriction digestion of cellular and plasmid DNA
B)  Production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs
C)  Separation of recombinant DNAs by electrophoresis using Southern blotting to determine where the desired recombinant molecule migrates
D)  Transformation of bacteria by the recombinant DNA plasmids using a selective medium
E)  Probing recombinant clones with a labeled DNA probe to find the desired cloneF) C) and D)
G) A) and E)

Question 7

Which of the following BEST describes knockout mice?

Accepted Answer

A)  They have a gene of interest that has been fully disabled.
B)  They have lower expression levels of a gene of interest.
C)  They have higher expression levels of a gene of interest.
D)  They have a point mutation in the gene of interest.
E)  They have a gene removed which results in lowered fertility.F) A) and E)
G) C) and D)

Question 8

The restriction enzyme Pst1 cleaves phosphodiester bonds between the G and the A nucleotides in its recognition sequence which is 5' CTGCAG 3' for one of the two DNA strands.After cutting by Pst1, the resulting DNA fragments will have:

Accepted Answer

A)  3' single-stranded overhanging ends (end in a 3' group) .
B)  5' single-stranded overhanging ends.
C)  blunt ends.
D)  3' P groups.
E)  both 5'single-stranded overhanging ends and 3' P groups.F) C) and D)
G) B) and D)

Question 9

Southern blotting is a technique used to transfer _____ to a solid medium.

Accepted Answer

A)  DNA
B)  RNA
C)  protein
D)  Two of the answers are correct.
E)  All of the answers are correct.F) A) and B)
G) C) and D)

Question 10

Before sequencing, the DNA fragment was cloned into a plasmid.On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was CLOSEST to the primer?

Accepted Answer

A)  G
B)  A
C)  C
D)  T
E)  G or TF) A) and D)
G) A) and E)

Question 11

You are interested in a particular segment of rhinoceros DNA and would like to clone it into a cloning plasmid.You have the following restriction map of the region that includes the DNA of interest and the plasmid (E = EcoRI, H = HindIII, X = XbaI, S = SphI, N = NotI) .  Which restriction enzymes would you choose to clone the DNA of interest into the cloning vector?

Accepted Answer

A)  E and H
B)  S
C)  X and N
D)  S and N
E)  S and XF) B) and C)
G) A) and E)

Question 12

Which of the following is NOT true regarding the differences between a genomic and cDNA library?

Accepted Answer

A)  Genomic libraries contain fewer restriction enzyme sites, whereas cDNA libraries contain many more.
B)  A genomic library is prepared from total genomic DNA, whereas a cDNA library is prepared from mRNA.
C)  Genomic libraries contain much more sequence information and are much larger than cDNA libraries.
D)  Genomic libraries contain coding and noncoding (regulatory, intron, etc.) sequences, whereas cDNA libraries contain only coding sequences along with their associated 5' and 3' untranslated regions.
E)  cDNA libraries are generated from mRNAs, whereas genomic libraries are not.F) B) and E)
G) A) and E)

Question 13

Restriction site sequences for six restriction enzymes are shown below.The "|" symbol shows the site at which the sequence is cut."N" is any nucleotide, "Pu" is any purine, and "Py" is any pyrimidine.Which of these enzymes would create blunt ends rather than "sticky" single-stranded ends?

Accepted Answer

Question 14

Which of the following would be MOST appropriate for cloning a gene that is 225 kb in size?

Accepted Answer

A)  Plasmid
B)  Cosmid
C)  Phage lambda
D)  BAC
E)  Yeast phageF) A) and B)
G) B) and E)

Question 15

One technique for finding a gene of interest involves first generating a genetic map to find the general location of the gene, and then identifying the specific location of the gene.What is this technique called?

Accepted Answer

A)  Next-generation sequencing
B)  DNA fingerprinting
C)  Positional cloning
D)  In silico gene discovery
E)  Site-directed mutagenesisF) B) and D)
G) All of the above

Question 16

Which of the following is NOT true regarding the basic components required for a bacterial cloning vector?

Accepted Answer

A)  Selectable markers provide a means for preferentially allowing growth of only those bacterial cells that have been transformed with the cloning vector.
B)  Unique restriction enzyme sites allow for pieces of foreign DNA to be inserted into the bacterial plasmid cloning vector that range in size from 20 kb to 50 kb.
C)  Unique restriction enzyme sites provide a means for inserting the foreign DNA into the cloning vector at a specific, known, sequence site.
D)  A bacterial origin of replication ensures that the plasmid is replicated while present within the bacterial cell.
E)  Selectable markers provide a means for selecting cells that have been transformed with a recombinant plasmid.F) B) and C)
G) C) and E)

Question 17

Which of the following is false of DNA sequences within a cDNA library?

Accepted Answer

A)  They do not contain exons.
B)  They do not contain introns.
C)  They do not contain promoters.
D)  They do not contain enhancers.
E)  They are produced from mRNAs.F) B) and C)
G) A) and D)

Question 18

Cronin et al.(Science, 2009, 325: 340-343)used RNA interference to study the immune response of the fruit fly, Drosophila melanogaster, to the bacterial pathogen, Serratia marcescens.They identified several members of the JAK-STAT cell-cell signaling pathway in their analysis.
a.The PIAS gene is a negative regulator of JAK-STAT signaling (that is, it inhibits the signaling pathway).RNAi to block PIAS activity causes flies to die significantly earlier than control flies when exposed to bacteria.Does JAK-STAT signaling appear to protect the flies from the bacteria? Explain.
b.The upd gene encodes a ligand that activates JAK-STAT signaling.RNAi that blocks upd function causes flies to survive longer than control flies when exposed to bacteria.Explain how this is or is not consistent with your answer from part a.

Accepted Answer

1)Pharmaceutical drugs (e.g., human insu...

Question 19

Which of the following remains a problem of gene therapy?

Accepted Answer

A)  To date, there has not been a case of gene therapy curing a disease.
B)  We have yet to develop a vector for delivering the gene.
C)  Patients mount immune responses to the transferred gene and vectors.
D)  We do not have the ability to use somatic gene therapy at this time.
E)  We can only alter germ-line cells at this time.F) B) and C)
G) All of the above

Question 20

A scientist mutates a gene in yeast and then looks to see what effect the mutation has on the phenotype in yeast.This is an example of:

Accepted Answer

A)  DNA fingerprinting.
B)  next-generation sequencing.
C)  transgenic research.
D)  reverse genetics.
E)  positional cloning.F) B) and E)
G) C) and D)

Exam 14: Molecular Genetic Analysis and Biotechnology

How might RNAi be used to treat diseases in humans?

Correct Answer
verified

Correct Answer
verified

Which of the following can be used for genetic engineering in plants?

Correct Answer
verified

All of the following are requirements of a bacterial cloning vector EXCEPT:

Correct Answer
verified

One technique for finding a gene of interest involves first generating a genetic map to find the general location of the gene, and then identifying the specific location of the gene.What is this technique called?

Correct Answer
verified

Southern blotting is a technique used to transfer _____ to a solid medium.

Correct Answer
verified

Restriction site sequences for six restriction enzymes are shown below.The "|" symbol shows the site at which the sequence is cut."N" is any nucleotide, "Pu" is any purine, and "Py" is any pyrimidine.Which of these enzymes would create blunt ends rather than "sticky" single-stranded ends?

Correct Answer
verified

Which of the following would be MOST appropriate for cloning a gene that is 225 kb in size?

Correct Answer
verified

A scientist mutates a gene in yeast and then looks to see what effect the mutation has on the phenotype in yeast.This is an example of:

Correct Answer
verified

Which of the following is NOT true regarding the basic components required for a bacterial cloning vector?

Correct Answer
verified

Which of the following is NOT part of the normal process of cloning recombinant DNA in bacteria?

Correct Answer
verified

A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site.After dideoxy sequencing, the gel pattern shown in this diagram is obtained.What was the sequence of the DNA strand that acted as the template in the sequencing reaction?

Correct Answer
verified

Before sequencing, the DNA fragment was cloned into a plasmid.On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was CLOSEST to the primer?

Correct Answer
verified

Which of the following BEST describes knockout mice?

Correct Answer
verified

The restriction enzyme Pst1 cleaves phosphodiester bonds between the G and the A nucleotides in its recognition sequence which is 5' CTGCAG 3' for one of the two DNA strands.After cutting by Pst1, the resulting DNA fragments will have:

Correct Answer
verified

Which of the following remains a problem of gene therapy?

Correct Answer
verified

Which of the following is NOT true regarding the differences between a genomic and cDNA library?

Correct Answer
verified

Correct Answer
verified
1)Pharmaceutical drugs (e.g., human insu...
View Answer

Correct Answer
verified

Which of the following is false of DNA sequences within a cDNA library?

Correct Answer
verified

Exam 14: Molecular Genetic Analysis and Biotechnology

How might RNAi be used to treat diseases in humans?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which of the following can be used for genetic engineering in plants?

Correct AnswerverifiedShow Answer

All of the following are requirements of a bacterial cloning vector EXCEPT:

Correct AnswerverifiedShow Answer

One technique for finding a gene of interest involves first generating a genetic map to find the general location of the gene, and then identifying the specific location of the gene.What is this technique called?

Correct AnswerverifiedShow Answer

Southern blotting is a technique used to transfer _____ to a solid medium.

Correct AnswerverifiedShow Answer

Restriction site sequences for six restriction enzymes are shown below.The "|" symbol shows the site at which the sequence is cut."N" is any nucleotide, "Pu" is any purine, and "Py" is any pyrimidine.Which of these enzymes would create blunt ends rather than "sticky" single-stranded ends?

Correct AnswerverifiedShow Answer

Which of the following would be MOST appropriate for cloning a gene that is 225 kb in size?

Correct AnswerverifiedShow Answer

A scientist mutates a gene in yeast and then looks to see what effect the mutation has on the phenotype in yeast.This is an example of:

Correct AnswerverifiedShow Answer

Which of the following is NOT true regarding the basic components required for a bacterial cloning vector?

Correct AnswerverifiedShow Answer

Which of the following is NOT part of the normal process of cloning recombinant DNA in bacteria?

Correct AnswerverifiedShow Answer

A fragment of DNA is cloned into a plasmid with a sequencing primer-binding site.After dideoxy sequencing, the gel pattern shown in this diagram is obtained.What was the sequence of the DNA strand that acted as the template in the sequencing reaction?

Correct AnswerverifiedShow Answer

Before sequencing, the DNA fragment was cloned into a plasmid.On the strand that acted as the template in the sequencing reaction, what base of the cloned fragment was CLOSEST to the primer?

Correct AnswerverifiedShow Answer

Which of the following BEST describes knockout mice?

Correct AnswerverifiedShow Answer

The restriction enzyme Pst1 cleaves phosphodiester bonds between the G and the A nucleotides in its recognition sequence which is 5' CTGCAG 3' for one of the two DNA strands.After cutting by Pst1, the resulting DNA fragments will have:

Correct AnswerverifiedShow Answer

Which of the following remains a problem of gene therapy?

Correct AnswerverifiedShow Answer

Which of the following is NOT true regarding the differences between a genomic and cDNA library?

Correct AnswerverifiedShow Answer

Correct Answerverified1)Pharmaceutical drugs (e.g., human insu...View AnswerShow Answer

Correct AnswerverifiedShow Answer

Which of the following is false of DNA sequences within a cDNA library?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
1)Pharmaceutical drugs (e.g., human insu...
View Answer

Correct Answer
verified

Correct Answer
verified